Consulta: creatorFacets:"Machado,Rosangela Zacarias"
Registros recuperados: 5
Data/hora: 18/05/2024 11:17:43

An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
Provedor de dados: Ciência Rural
Autores: Baldani,Cristiane Divan; Machado,Rosangela Zacarias; Botteon,Paulo de Tarso Landgraf; Takakura,Felipe Santoro; Massard,Carlos Luiz.
A crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Babesia equi; Equine piroplasmosis; ELISA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000500031

Apoptose na infecção experimental de cães domésticos com Ehrlichia canis
Provedor de dados: Ciência Rural
Autores: Sanchez,Irma Ximena Barbosa; Socarras,Teresa de Jesus Oviedo; Silva,Miguel Ladino; Pereira,Nubia Braga; Machado,Rosangela Zacarias; Vasconcelos,Anilton Cesar.
A Erliquiose canina é uma zoonose causada pela Ehrlichia canis, bactéria Gram negativa de distribuição mundial. Alguns cães com erliquiose se tornam portadores assintomáticos enquanto outros desenvolvem uma doença aguda com morte rápida. A apoptose pode ser importante na eliminação de patógenos intracelulares, podendo, nas infecções por Ehrlichia sp. e Anaplasma sp., ocorrer modulação da apoptose celular para prolongar a sobrevivência desses organismos. Para avaliação do papel da apoptose na erliquiose canina, sete cães foram distribuídos em dois grupos. No Grupo inoculado, realizou-se a infecção por via intravenosa com sangue infectado com E. canis (isolado Jaboticabal), sendo realizada a inoculação com PBS estéril nos animais pertencentes ao Grupo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptose; Ehrlichia canis; N-PCR; RIFI; TUNEL; Bcl-2; Bax.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800021

Canine ehrlichiosis: clinical, hematological, serological and molecular aspects
Provedor de dados: Ciência Rural
Autores: Nakaghi,Andréa Cristina Higa; Machado,Rosangela Zacarias; Costa,Mirela Tinucci; André,Marcos Rogério; Baldani,Cristiane Divan.
The aim of the present study was to compare the direct detection methods of Ehrlichia canis (blood smears and nested PCR), serological tests (Dot-ELISA and Immunofluorescent Antibody Test - IFAT), and demonstrate the most suitable test for the diagnosis of different stages of infection. Blood samples and clinical data were collected from 30 dogs examined at the Veterinary Teaching Hospital, UNESP, Jaboticabal, SP, Brazil. The clinical signs most frequently observed were apathy, anorexia, pale mucous membrane, fever, lymphadenopathy, splenomegaly, hemorrhages and uveitis. Evaluating the humoral immune response, 63.3% of the sera were IFAT positive, while 70% were Dot-ELISA positive. By nestedPCR 53.3% of the samples were positive. Comparing these techniques...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ehrlichia canis; Dog; IFAT; Dot-ELISA; Nested PCR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300027

Evaluation of dot-blot test for serological diagnosis of bovine brucellosis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Bastos,Carla Resende; Mathias,Luis Antonio; Jusi,Márcia Mariza Gomes; Santos,Renata Ferreira dos; Silva,Glaucenyra Cecília Pinheiro da; André,Marcos Rogério; Machado,Rosangela Zacarias; Bürger,Karina Paes.
Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator. At the end of standardization, it was established as optimal for the antigen obtained from Brucella abortus B19 after passing through a microorganism rupture process, the blood serum samples diluted at 1:100, and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucella abortus; Animal health; Zoonoses; 2-Mercaptoethanol; Complement fixation test.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300564

Serosurvey for canine visceral leishmaniasis in rural and urban areas of the Brazilian Legal Amazon
Provedor de dados: Brazilian Journal of Infectious Diseases
Autores: Guimarães,Andresa; Raimundo,Juliana Macedo; Santos,Helciléia Dias; Machado,Rosangela Zacarias; Baldani,Cristiane Divan.
Tipo: Info:eu-repo/semantics/other
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000200207